Gibberellic Acid
Sooner or later every grower is going to want to produce marijuana seeds. Developing a new stable strain is beyond the scope of this discussion and requires the ability to grow hundreds or even thousands of breeding plants. However, just about any grower can manage to preserve some genetics by growing f2 seeds where they have crossed a male and female of the same strain, or can produce a simple cross which would be referred to as strain1xstrain2 for instance white widow crossed with ak-47 would be referred to as a WW x AK-47. You can produce some excellent seed and excellent marijuana this way.
To Feminise or not to Feminise
There are numerous myths surrounding feminized seeds. Feminizing seeds is a bit more work than simply crossing two plants naturally. However it will save you a lot of time in the end. If you make fem seeds properly then there is no increased chance of hermaphrodites and all seeds will be female. This means no wasted time and effort growing males and it means that all your viable seeds produce useful plants, since roughly half of normal seeds are male this effectively doubles the number of seeds you have.
Other times you will have no choice but to produce feminized seed because it will be a female plants genetics that you want to preserve and you won’t have any males. Perhaps you received these genetics via clone or didn’t keep males.
The new thing on the market for commercial Cannabis cultivation are Autoflowering feminized strains. By crossing of the Cannabis ruderalis with Sativa and Indica strains many cultivators have created interesting hybrids which boast benefits from both sides of these families.
Although Sensi Seeds already created the Ruderalis Indica and the Ruderalis Skunk crossing, the first variety to be marketed specifically as ‘Autoflowering cannabis seed’ was the Lowryder #1. This ‘hybrid’ was a crossing between a Ruderalis, a William’s Wonder and a Northern Lights #2. This strain was marketed by ‘The Joint Doctor’ and was honestly speaking not very impressive. The genetics of the ruderalis was still highly present which caused for a very low yield and little psychoactive effect.
Despite these first disappointing results for the grower and user, the interest of the cannabis community was most definitely caught. After the Lowryder #1 the Lowryder #2 was introduced by ´The Joint Doctor´. See also the article:”What are autoflowering cannabis seeds” about auto-flowering seeds.
Auto-flowering cannabis and the easily distributed seed have opened a whole new market in the world of the online grow-shop, making it easy for home growers with shortage of space to grow rewarding cannabis plants in many different varieties.
Selecting Suitable Parents
There are a number of important characteristics when selecting parents. First are you making fem seeds? If you are then both parents will be female. This makes things easier. If not then the best you can do is select a male with characteristics in common with the females you hope to achieve from the seed.
Obviously potency, yield, and psychoactive effects are critical to the selection process. But some other important traits are size, odor, taste, resistance to mold and contaminants, early finishing and consistency.
Collecting and Storing PollenIn order to collect pollen you simply put down newspaper around the base of the plant. The pollen will fall from the plant onto the newspaper. You can then put this newspaper into a plastic bag and store it in the refrigerator or freeze it. Pollen will keep for a few months in the refrigerator and can be used on the next crop. The freezer will extend that to up to six months but gives the pollen a lower chance of viability that increases with time.
Pollinating a Plant
To pollinate a plant you can brush the pollen on a flower with a cotton swab or you can take the plastic bag and wrap the flower inside it and shake. In this way you can selectively pollinate plants and even individual buds and branches.
Male Isolation
A male plant or a plant with male flowers will pollinate your entire crop rendering it seedy. You probably don’t want THAT many seeds so how can you avoid it? Moving the male to another room might work but if that other room shares an air path via ducting or air conditioning then pollen may still find its way. One technique is to construct a male isolation chamber.
A male isolation chamber is simply a transparent container such as a large plastic storage tub turned on its side (available at your local megamart). Get a good sized PC fan that can be powered with pretty much any 12v wall adapter, by splicing together the + (yellow or red on fan, usually dotted on power adapter) and the – wires (black on fan, usually dotted power adapter) just twist with the like wire on the other device and then seal up the connection with electric tape. Then take a filtrate filter and cut out squares that fit the back of the pc fan so that the fan pulls (rather than pushes) air through the filter. Tape several layers of filter to the back of the pc fan so all the air goes through the filter. Now cut a large hole in the top of the plastic container and mount the pc fan over top of it so it pulls air out the box. You can use silicon sealant, latex, whatever you’ve got that gives a good tight seal.
This can be used as is, or you can cut a small intake in the bottom to improve airflow. Pollen won’t be able to escape the intake as long as the fan is moving but you might put filter paper over the intake to protect against fan failures. You can also use grommets to seal holes and run tubing into the chamber in order to water hydroponically from a reservoir outside the chamber. Otherwise you will need to remove the whole chamber to a safe location in order to water the plant or maintain a reservoir kept inside the chamber.
Making Feminised Seed
To make feminized seed you must induce male flowers in a female plant. There is all sorts of information on the Internet about doing this with light stress (light interruptions during flowering) and other forms of stress. The best of the stress techniques is to simply keep the plant in the flowering stage well past ripeness and it will produce a flower.
Stress techniques will work but whatever genetic weakness caused the plants to produce a male flower under stress will be carried on to the seeds. This means the resulting seeds have a known tendency to produce hermaphrodites. Fortunately, environmental stress is not the only way to produce male flowers in a female plant.
The ideal way to produce feminized seed through hormonal alteration of the plant. By adding or inhibiting plant hormones you can cause the plant to produce male flowers. Because you did not select a plant that produces male flowers under stress there is no genetic predisposition to hermaphroditism in the seed vs plants bred between a male and female parent. There are actually a few ways to do this, the easiest I will list here.
Colloidal Silver (CS)
This is the least expensive and most privacy conscious way to produce fem seed. CS has gotten a bad name because there is so much bad information spread around about its production and concentrations. It doesn’t help that there are those who believe in drinking low concentration colloidal silver for good health and there is information mixed in about how to produce that low concentration food grade product. Follow the information here and you will consistently produce effective CS and know how to apply it to get consistent results.
Simply construct a generator using a 9-12v power supply (DC output, if it says AC then its no good) that can deliver at least 250ma (most wall wart type power supplies work, batteries are not recommended since their output varies over time). The supply will have a positive and negative lead, attach silver to each lead (contrary to Internet rumors, you aren’t drinking this is cheap 925 silver is more than pure enough) you can expose the leads by clipping off the round plug at the end and splitting the wires, one will be positive and the other negative just like any old battery. Submerge both leads about 2-3 inches apart in a glass of distilled water (roughly 8oz). Let this run for 8-24hrs (until the liquid reads 12-15ppm) and when you return the liquid will be a purple or silver hue and there may be some precipitate on the bottom.
This liquid is called colloidal silver. It is nothing more or less than fine particles of silver suspended in water so it is a completely natural solution and is safe to handle without any special precautions. The silver inhibits female flowering hormones in cannabis and so the result is that male flowering hormone dominates and male flowers are produced.
To use the silver, spray on a plant or branch three days prior to switching the lights to 12/12 and continue spraying every three days until you see the first male flowers. Repeated applications after the first flowers appear may result in more male flowers and therefore more pollen. As the plant matures it will produce pollen that can be collected and used to pollinate any female flower (including flowers on the same plant).
Silver Thiosulfate (STS)
Only mentioned for completeness. Silver Thiosulfate is more difficult to acquire and works on the same principle as CS. Its application is similar to CS and achieves the same results.
Gibberellic Acid (GA3)
This is probably the most popular way to produce feminized seed. GA3 can be purchased readily in powdered form, a quick search reveals numerous sources on e-bay for as little as $15. Simply add to water to reach 100ppm concentration and spray the plant daily for 10 days during flowering and male flowers will be produced.
Article: Marijuana Cultivation/Producing Seeds http://en.wikibooks.org/wiki/Marijuana_Cultivation/Producing_Seeds
Female Cannabis Seeds 